Regulatory

Part:BBa_K1484347:Design

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)


P_SuI2, marchantia promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 429
    Illegal SpeI site found at 1607
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1471
    Illegal SpeI site found at 429
    Illegal SpeI site found at 1607
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 236
    Illegal BglII site found at 1003
    Illegal BamHI site found at 1462
    Illegal XhoI site found at 1040
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 429
    Illegal SpeI site found at 1607
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 429
    Illegal SpeI site found at 1607
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1891


Design Notes

The removal of illegal restriction sites was considered but not completed due to direct interaction of the DNA with regulatory proteins. Also, it was ensured that the region selected for this part was directly upstream of the first ATG of the gene used to identify the promoter sequence. This was so that the 5' UTR, that is vital in plants, was maintained.


Source

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription under conditions of sulphur limitation. We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.


References

Nussaume L et al. 2011. Phosphate Import in Plants: Focus on the PHT1 Transporters. Front Plant Sci. 2: 83. Schachtman D et al. 1998. Phosphorus Uptake by Plants: From Soil to Cell. Plant Physiology 116(2): 447-453. TAIR Locus: AT2G38940 ThaleMine ATG id: AT2G16430